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pecfp c1 vector  (Addgene inc)


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    Addgene inc pecfp c1 vector
    Pecfp C1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pecfp+vectors/pFRET-HSP33+cys+(Plasmid+%2316076)/pmc12074641-190-11-19
    Average 93 stars, based on 10 article reviews
    pecfp c1 vector - by Bioz Stars, 2026-09
    93/100 stars

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    Construct:

    Article Title: Transmembrane helix connectivity in Orai1 controls two gates for calcium-dependent transcription
    Article Snippet: N-terminally tagged Orai1 constructs (accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_032790.3","term_id":"170932551","term_text":"NM_032790.3"}} NM_032790.3 ) were cloned into the SalI and SmaI restriction sites of pECFP-C1 and pEYFP-C1 expression vectors (Clontech). .. GFP-TFEB and GFP-MITF were purchased from Addgene and subcloned into peCFP vectors. hOrai1 Δ1-64, N223A, and cysteine-free (C126V, C143V, C195V) constructs were used for crosslinking as described in ( 12 ). .. Point mutations in Orai1 constructs were introduced using the QuikChange site-directed mutagenesis kit (Stratagene).



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